Compositions and methods for micronutrient introduction

ABSTRACT

The present disclosure provides compositions comprising a ligand and a fertilizer, including kits comprising the ligand. The disclosure also provides method of growing a plant, wherein the method comprises the step of contacting the plant with a composition comprising a ligand.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional application of U.S. patent applicationSer. No. 15/528,645, filed on May 22, 2017, which is the U.S. nationalphase of PCT/US2015/062948, filed on Nov. 30, 2015, and claims thebenefit under 35 USC § 119(e) of U.S. Provisional Application Ser. No.62/085,969, filed on Dec. 1, 2014, the entire disclosures of which areincorporated herein by reference.

TECHNICAL FIELD

The invention described herein pertains to composition and methods, anduses thereof, for introducing micronutrients into plants. In particular,the invention described herein pertains to compounds including a ligandand optionally a fertilizer, and methods and uses thereof, for improvingagricultural productivity.

BACKGROUND AND SUMMARY OF THE INVENTION

The production and development of plants and plant products foragricultural use are important concerns for the growing world. As plantproducts are utilized not only as human foods but also as animal feedsand as fuels, and given that the worldwide human population has beenestimated to exceed 9 billion people by the year 2050, the necessity toincrease the efficiency and productivity of agricultural processes ismore important than ever.

Modern agriculture has developed a number of processes to assist withincreasing productivity of plants and plant products. For example, theuse of improved mechanization, advanced fertilizers, and high-qualityhybrid seeds can all contribute to increasing productivity by improvingthe yield of plants grown per unit of land. However, despite theseadvances, new solutions to solve the problems of producing anddeveloping plant products are still needed to further improveagricultural productivity.

Accordingly, the present disclosure provides compositions and methodscomprising ligands that exhibit desirable properties and provide relatedadvantages for improving agricultural productivity.

The compositions and methods utilizing compositions comprising a ligandaccording to the present disclosure provide several advantages comparedto other methods known in the art. First, the compositions comprising aligand are effective to increase yield of a plant (e.g., number ofbushels) produced per acre of land. Second, the compositions comprisinga ligand are able to employ existing micronutrients in the soil that aretypically underutilized by plants. It is believed that the compositionscomprising a ligand are able to chelate with the existingmicronutrients, allowing the micronutrients to be available for uptakeby the plants.

Third, the compositions are able to chelate micronutrients in afertilizer formulation that are typically not able to be fully chelated,thus improving the availability of the micronutrients for plant uptake.For example, the fertilizer formulation can be a tank mixed formulationthat can be easily employed for utilization by the end user.

Fourth, by chelating with micronutrients in the soil or in fertilizerformulations, the compositions prevent the micronutrients frominteracting with phosphorus, thereby indirectly increasing theavailability of phosphorus to plants. Furthermore, the micronutrientsare able to more efficiently enter the plant and are transported to thecellular level, thus assuring their availability to the plant cells.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 shows the yield response (bushels/acre) to starter fertilizertreatment groups, sorted by yield level.

FIG. 2 shows the plant tissue phosphorus (P) response (percentagepresent in ear leaf) to starter fertilizer treatment groups, sorted byyield level.

FIG. 3 shows the grain (P) removal response (pounds of P2O5/acre) tostarter fertilizer treatment groups, sorted by yield level.

FIG. 4 shows the effects of ligand (WC101) treatment on the percentageof copper (Cu) recovery 35 days after its addition to the threedifferent soil types.

FIG. 5 shows the effects of ligand (WC101) treatment on the percentageof iron (Fe) recovery 35 days after its addition to the three differentsoil types.

FIG. 6 shows the effects of ligand (WC101) treatment on the percentageof manganese (Mn) recovery 35 days after its addition to the threedifferent soil types.

FIG. 7 shows the effects of ligand (WC101) treatment on the percentageof zinc (Zn) recovery 35 days after its addition to the three differentsoil types.

FIG. 8 shows the effects of ligand (WC101) treatment on the percentageof phosphorus (P) recovery 35 days after its addition to the threedifferent soil types. Mean values with the same letter are notstatistically different (Fisher's LSD, α=0.05).

FIG. 9 shows that plot unit sizes were approximately 10 feet by 40 feetaccording to the disclosed layout.

The following numbered embodiments are contemplated and arenon-limiting:

1. A composition comprising a ligand and a fertilizer.

2. The composition of clause 1 wherein the composition is substantiallyfree of iron.

3. The composition of clause 1 or clause 2 wherein the composition issubstantially free of a metal.

4. The composition of clause 3 wherein the ligand is a metal chelatingagent.

5. The composition of clause 3 wherein the metal is iron.

6. The composition of any of clauses 1 to 5 wherein the ligand isnon-chelated.

7. The composition of any of clauses 1 to 6 wherein the ligand issubstantially free of a metal.

8. The composition of clause 7 wherein the ligand is a metal chelatingagent.

9. The composition of clause 8 wherein the metal is iron.

10. The composition of any of clauses 1 to 9 wherein the ligand is ofthe formula

or a salt thereof,wherein

R^(a) is independently selected in each instance from the groupconsisting of H, —CH₂CO₂H or a salt thereof, and —CH₂CH₂OH;

R^(b) is independently selected in each instance from the groupconsisting of —CH₂CH₂OH, —CH₂CO₂H, or a salt thereof, and

wherein the asterisk indicates the point of attachment, X isindependently selected in each instance from the group consisting of H,—CO₂H, or a salt thereof, and —CH₂OH;

R^(Ar) represents from 0 to 2 substituents independently selected ineach instance from the group consisting of F, Cl, —OH, —NH₂, —CH₃, —CN,—CO₂H, or a salt thereof, —C(O)NH₂, —SO₃H, or a salt thereof, —SO₂NH₂,—PO₃H₂, or a salt thereof, and OR¹, where R¹ is C₁-C₄ alkyl; and

the ligand includes two or more carboxylic acid groups other thanR^(Ar).

11. The composition of clause 10 wherein each R^(a) is identical andeach R^(b) is identical.

12. The composition of clause 10 or 11 wherein R^(a) is not identical toR^(b).

13. The composition of clause 10 or 11 wherein R^(a) is identical toR^(b).

14. The composition of any of clauses 1 to 13 wherein the ligand isethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid) (EDDHA).

15. The composition of any of clauses 1 to 14 wherein the ligand isortho-ortho EDDHA.

16. The composition of any of clauses 1 to 15 wherein the compositionfurther comprises zinc.

17. The composition of clause 16 wherein the zinc is chelated zinc.

18. The composition of any of clauses 1 to 17 wherein the compositionfurther comprises a herbicide.

19. The composition of any of clauses 1 to 18 wherein the compositionfurther comprises a water conditioning agent.

20. The composition of any of clauses 1 to 19 wherein the compositionfurther comprises a micronutrient product.

21. The composition of any of clauses 1 to 20 wherein the fertilizer isan ammonium polyphosphate fertilizer.

22. The composition of clause 21 wherein the ammonium polyphosphatefertilizer is ammonium polyphosphate 10-34-0 fertilizer.

23. The composition of any of clauses 1 to 22 wherein the herbicide isglyphosate.

24. A composition consisting essentially of a ligand and a fertilizer.

25. The composition of any of clauses 1 to 24 wherein the ligand isnon-chelated.

26. The composition of any of clauses 1 to 25 wherein the fertilizer isan ammonium polyphosphate fertilizer.

27. The composition of clause 26 wherein the ammonium polyphosphatefertilizer is ammonium polyphosphate 10-34-0 fertilizer.

28. A method of growing a plant, said method comprising the step ofcontacting the plant with a composition comprising a ligand.

29. The method of clause 28 wherein the yield of the plant is improvedvia contacting the plant with the composition.

30. The method of clause 28 or clause 29 wherein the composition issubstantially free of iron.

31. The method of any of clauses 28 to 30 wherein the composition issubstantially free of a metal.

32. The method of any of clauses 28 to 31 wherein the ligand is a metalchelating agent.

33. The method of clause 32 wherein the metal is iron.

34. The method of any of clauses 28 to 33 wherein the ligand isnon-chelated.

35. The method of any of clauses 28 to 34 wherein the ligand issubstantially free of a metal.

36. The method of clause 35 wherein the ligand is a metal chelatingagent.

37. The method of clause 36 wherein the metal is iron.

38. The method of any of clauses 28 to 37 wherein the ligand is of theformula

or a salt thereof,wherein

R^(a) is independently selected in each instance from the groupconsisting of H, —CH₂CO₂H or a salt thereof, and —CH₂CH₂OH;

R^(b) is independently selected in each instance from the groupconsisting of —CH₂CH₂OH, —CH₂CO₂H, or a salt thereof, and

wherein the asterisk indicates the point of attachment, X isindependently selected in each instance from the group consisting of H,—CO₂H, or a salt thereof, and —CH₂OH;

R^(Ar) represents from 0 to 2 substituents independently selected ineach instance from the group consisting of F, Cl, —OH, —NH₂, —CH₃, —CN,—CO₂H, or a salt thereof, —C(O)NH₂, —SO₃H, or a salt thereof, —SO₂NH₂,—PO₃H₂, or a salt thereof, and OR¹, where R¹ is C₁-C₄ alkyl; and

the ligand includes two or more carboxylic acid groups other thanR^(Ar).

39. The method of clause 38 wherein each R^(a) is identical and eachR^(b) is identical.

40. The method of clause 38 or 39 wherein R^(a) is not identical toR^(b).

41. The method of clause 38 or 39 wherein R^(a) is identical to R^(b).

42. The method of any of clauses 28 to 41 wherein the ligand isethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid) (EDDHA).

43. The method of any of clauses 28 to 42 wherein the ligand isortho-ortho EDDHA.

44. The method of any of clauses 28 to 43 wherein the ligand isnon-chelated.

45. The method of any of clauses 28 to 44 wherein the compositionfurther comprises a fertilizer.

46. The method of any of clauses 28 to 45 wherein the compositionfurther comprises zinc.

47. The method of clause 46 wherein the zinc is chelated zinc.

48. The method of any of clauses 28 to 47 wherein the compositionfurther comprises a herbicide.

49. The method of any of clauses 28 to 48 wherein the compositionfurther comprises a water conditioning agent.

50. The method of any of clauses 28 to 49 wherein the compositionfurther comprises a micronutrient product.

51. The method of any of clauses 28 to 50 wherein the compositionconsists essentially of the ligand and the fertilizer.

52. The method of any of clauses 28 to 51 wherein the fertilizer is anammonium polyphosphate fertilizer.

53. The method of clause 52 wherein the ammonium polyphosphatefertilizer is ammonium polyphosphate 10-34-0 fertilizer.

54. The method of any of clauses 28 to 53 wherein the herbicide isglyphosate.

55. The method of any of clauses 28 to 54 wherein the plant is a seed.

56. The method of any of clauses 28 to 55 wherein the plant is corn.

57. The method of any of clauses 28 to 55 wherein the plant is soybean.

58. A kit comprising a ligand and instructions for combining the ligandwith a fertilizer.

59. The kit of clause 58 wherein the ligand is non-chelated.

60. The kit of clause 58 or clause 59 wherein the ligand issubstantially free of a metal.

61. The kit of clause 60 wherein the ligand is a metal chelating agent.

62. The kit of clause 60 wherein the metal is iron.

63. The kit of any of clauses 58 to 62 wherein the ligand is of theformula

or a salt thereof,wherein

R^(a) is independently selected in each instance from the groupconsisting of H, —CH₂CO₂H or a salt thereof, and —CH₂CH₂OH;

R^(b) is independently selected in each instance from the groupconsisting of —CH₂CH₂OH, —CH₂CO₂H, or a salt thereof, and

wherein the asterisk indicates the point of attachment, X isindependently selected in each instance from the group consisting of H,—CO₂H, or a salt thereof, and —CH₂OH;

R^(Ar) represents from 0 to 2 substituents independently selected ineach instance from the group consisting of F, Cl, —OH, —NH₂, —CH₃, —CN,—CO₂H, or a salt thereof, —C(O)NH₂, —SO₃H, or a salt thereof, —SO₂NH₂,—PO₃H₂, or a salt thereof, and OR¹, where R¹ is C₁-C₄ alkyl; and

the ligand includes two or more carboxylic acid groups other thanR^(Ar).

64. The kit of clause 63 wherein each R^(a) is identical and each R^(b)is identical.

65. The kit of clause 63 or 64 wherein R^(a) is not identical to R^(b).

66. The kit of clause 63 or 64 wherein R^(a) is identical to R^(b).

67. The kit of any of clauses 58 to 66 wherein the ligand isethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid) (EDDHA).

68. The kit of any of clauses 58 to 67 wherein the ligand is ortho-orthoEDDHA.

69. The kit of any of the above clauses wherein the composition furthercomprises zinc.

70. The kit of clause 69 wherein the zinc is chelated zinc.

71. The kit of any of clauses 58 to 70 wherein the composition furthercomprises a herbicide.

72. The kit of any of clauses 58 to 71 wherein the composition furthercomprises a water conditioning agent.

73. The kit of any of clauses 58 to 72 wherein the composition furthercomprises a micronutrient product.

74. The kit of any of clauses 58 to 73 wherein the fertilizer is anammonium polyphosphate fertilizer.

75. The kit of clause 74 wherein the ammonium polyphosphate fertilizeris ammonium polyphosphate 10-34-0 fertilizer.

76. The kit of any of clauses 58 to 75 wherein the herbicide isglyphosate.

DETAILED DESCRIPTION

Various aspects of the invention are described herein as follows. In oneaspect described herein, a composition comprising a ligand and afertilizer is provided. In another aspect, a composition consistingessentially of a ligand and a fertilizer is provided. In yet anotheraspect, a method of growing a plant is provided. The method comprisesthe step of contacting the plant with a composition comprising a ligand.In another aspect, a kit is provided. The kit comprises a ligand andinstructions for combining the ligand with a fertilizer.

In some aspects, a composition comprising a ligand and a fertilizer isprovided. As used herein, the term “ligand” refers to a chelating agentcapable of forming chelation compounds with various substances. In someembodiments, the ligand is selected from a group consisting ofethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid) (EDDHA),ethylenediaminedi(2-hydroxy-5-sulfophenylacetic) acid (EDDHSA),ethylenediaminetetraacetic acid (EDTA),N-{2-[bis(carboxymethyl)amino]ethyl}-N-(2-hydroxyethyl)glycine (HEDTA),N,N-Bis{2-[bis(carboxymethyl)amino]ethyl}glycine (DTPA),ethylenediaminedi(o-hydroxy-p-methylphenylacetic) acid (EDDHMA),ethylenediaminedi(5-carboxy-2-hydroxyphenylacetic) acid (EDDCHA),[Ethylenebis(2-hydroxybenzylimino)]diacetic acid (HBED), andglucoheptonate (GH).

In various embodiments, the ligand is of the formula

or a salt thereof,wherein

R^(a) is independently selected in each instance from the groupconsisting of H, —CH₂CO₂H or a salt thereof, and —CH₂CH₂OH;

R^(b) is independently selected in each instance from the groupconsisting of —CH₂CH₂OH, —CH₂CO₂H, or a salt thereof, and

wherein the asterisk indicates the point of attachment, X isindependently selected in each instance from the group consisting of H,—CO₂H, or a salt thereof, and —CH₂OH;

R^(Ar) represents from 0 to 2 substituents independently selected ineach instance from the group consisting of F, Cl, —OH, —NH₂, —CH₃, —CN,—CO₂H, or a salt thereof, —C(O)NH₂, —SO₃H, or a salt thereof, —SO₂NH₂,—PO₃H₂, or a salt thereof, and OR¹, where R¹ is C₁-C₄ alkyl; and

the ligand includes two or more carboxylic acid groups other thanR^(Ar). In some embodiments of the composition, each R^(a) is identicaland each R^(b) is identical in the ligand. In other embodiments of thecomposition, R^(a) is not identical to R^(b) in the ligand. In yet otherembodiments of the composition, R^(a) is identical to R^(b) in theligand.

In various embodiments, the ligand is EDDHA. The chemical structure ofEDDHA is:

In some embodiments, the ligand is ortho-ortho EDDHA. The chemicalstructure of ortho-ortho EDDHA is:

In some embodiments, the ligand is EDDHSA. In other embodiments, theligand is EDTA. In yet other embodiments, the ligand is HEDTA. In someembodiments, the ligand is DTPA. In other embodiments, the ligand isEDDHMA. In yet other embodiments, the ligand is EDDCHA. In someembodiments, the ligand is HBED. In other embodiments, the ligand is GH.

As used herein, the term “fertilizer” refers to any organic or inorganicmaterial, of natural or of synthetic origin, which may be added to soilto supplement one or more plant nutrients that aid in the growth ofplants. As known to the skilled artisan, a fertilizer can be customblended according to the need and desire of a plant producer. Fertilizerblends comprising various combinations of elements and materials can beeasily envisioned by the skilled artisan.

In various embodiments, the fertilizer is an ammonium polyphosphatefertilizer. The term “ammonium phosphate fertilizer” can comprise any ofa wide variety of phosphates, such as orthophosphate, pyrophosphate,tripolyphosphate, tetrapolyphosphate, trimetaphosphate, and the like,and mixtures thereof, and one or more ammonium counterions. In someembodiments, the phosphate comprises a polyphosphate. In otherembodiments, the phosphate comprises an orthophosphate. In someembodiments, the ammonium phosphate fertilizer comprises monoammoniumphosphate. In other embodiments, the ammonium phosphate fertilizercomprises diammonium phosphate. In yet other embodiments, the ammoniumphosphate fertilizer comprises ammonium polyphosphate. In otherembodiments, the ammonium phosphate fertilizer comprises ammoniumorthophosphate. In some embodiments, the fertilizer is a liquidfertilizer. In other embodiments, the fertilizer is a non-liquidfertilizer.

In some embodiments, the fertilizer is a fertilizer selected from thegroup consisting of 10-34-0, 7-21-7, and 4-10-10. In some embodiments,the fertilizer is derived from 10-34-0 fertilizer further comprisingsoluble potash (for supplying potassium (K)). In other embodiments, thefertilizer is a fertilizer selected from the group consisting of 9-18-9,6-24-6, and 4-18-18. In some embodiments, the fertilizer is derived fromphosphoric acid. In yet other embodiments, the fertilizer is afertilizer selected from the group consisting of 7-23-3, 7-25-5, and6-22-10. In some embodiments, the fertilizer is produced from acombination of high ortho acid and high poly acid. In one embodiment,the fertilizer is 10-34-0. In another embodiment, the fertilizer is7-21-7. In yet another embodiment, the fertilizer is 4-10-10. In oneembodiment, the fertilizer is 9-18-9. In another embodiment, thefertilizer is 6-24-6. In yet another embodiment, the fertilizer is4-18-18. In one embodiment, the fertilizer is 7-23-3. In anotherembodiment, the fertilizer is 7-25-5. In yet another embodiment, thefertilizer is 6-22-10.

In some embodiments, the composition is substantially free of iron. Inother embodiments, the composition is substantially free of a metal.

In certain embodiments, the ligand is a metal chelating agent. In someembodiments, the metal is iron.

In other embodiments, the ligand is non-chelated. As used herein, theterm “chelated” or “chelation” refers to the formation or presence ofone or more bonds, or other attractive interactions, between two or moreseparate binding sites within a ligand and a single central atom.

In yet other embodiments, the ligand is substantially free of a metal.In some embodiments, the ligand is a metal chelating agent. In certainembodiments, the metal is iron.

In certain embodiments, the composition further comprises zinc. In someembodiments, the zinc is chelated zinc. Any zinc-containing product canbe used according to these embodiments. In some embodiments, zinc may beprovided by one or more of the following zinc-containing products:Nortrace EDTA 9% Zinc, Foli-Gro Zinc 10%, Pro-Zinc 10, RNA EDTA 9% Zinc,Tracite LF Zinc 10%, Ultra Che Zinc 9% EDTA, Zinc Gro 10 Liquid, CitriChe Zinc 10% EDTA. In one embodiment, zinc is provided by Nortrace EDTA9% Zinc. In another embodiment, zinc is provided by Foli-Gro Zinc 10%.In yet another embodiment, zinc is provided by Pro-Zinc 10. In oneembodiment, zinc is provided by RNA EDTA 9% Zinc. In another embodiment,zinc is provided by Tracite LF Zinc 10%. In yet another embodiment, zincis provided by Ultra Che Zinc 9% EDTA. In one embodiment, zinc isprovided by Zinc Gro 10 Liquid. In another embodiment, zinc is providedby Citri Che Zinc 10% EDTA.

In various embodiments, the composition further comprises a herbicide.As used herein, the term “herbicide” refers to a molecule or combinationof molecules that retards or otherwise kills undesirable, unwantedplants; such as, but not limited to, deleterious or annoying weeds,broadleaf plants, grasses, and sedges; and may be used in this mannerfor crop protection. Numerous herbicides are known in the art including,for example, those available athttp://www.alanwood.net/pesticides/class_pesticides.html. In variousembodiments, the herbicide is selected from the group consisting of2,4-D, 2,4-DB, acetochlor, acifluorfen, alachlor, ametryn, atrazine,aminopyralid, benefin, bensulfuron, bensulide, bentazon, bromacil,bromoxynil, butylate, carfentrazone, chlorimuron, chlorsulfuron,clethodim, clomazone, clopyralid, cloransulam, cycloate, DCPA,desmedipham, dicamba, dichlobenil, diclofop, diclosulam, diflufenzopyr,dimethenamid, diquat, diuron, DSMA, endothall, EPTC, ethalfluralin,ethofumesate, fenoxaprop, fluazifop-P, flucarbazone, flufenacet,flumetsulam, flumiclorac, flumioxazin, fluometuron, fluroxypyr,fomesafen, foramsulfuron, glufosinate, glyphosate, halosulfuron,hexazinone, imazamethabenz, imazamox, imazapic, imazaquin, imazethapyr,isoxaben, isoxaflutole, lactofen, linuron, MCPA, MCPB, mesotrione,metolachlor-s, metribuzin, metsulfuron, molinate, MSMA, napropamide,naptalam, nicosulfuron, norflurazon, oryzalin, oxadiazon, oxyfluorfen,paraquat, pelargonic acid, pendimethalin, phenmedipham, picloram,primisulfuron, prodiamine, prometryn, pronamide, propanil, prosulfuron,pyrazon, pyrithiobac, quinclorac, quizalofop, rimsulfuron, sethoxydim,siduron, simazine, sulfentrazone, sulfometuron, sulfosulfuron,tebuthiuron, terbacil, thiazopyr, thifensulfuron, thiobencarb,tralkoxydim, triallate, triasulfuron, tribenuron, triclopyr,trifluralin, triflusulfuron, and any combination thereof. In someembodiments, the herbicide is glyphosate.

In some embodiments, the composition further comprises a waterconditioning agent. As used herein, the term “water conditioning agent”refers to any substance that improves or enhances herbicide and/orpesticide performance. For example, a water conditioning agent canenhance pesticide performance by modifying factors such as pH and hardwater, enhance herbicide uptake by plants, and improve pesticide sprayapplication and efficacy.

In other embodiments, the composition further comprises a micronutrientproduct. For example, micronutrient products can include trace elementssuch as copper, iron, manganese, zinc, cobalt, molybdenum, and/or boron.These trace elements can be referred to as “micronutrients” because ofthe relatively small amounts required by plants for growth. In someembodiments, a micronutrient product is selected from Max In Ultra ZMB,First Choice Foliar Nutrient, Foli Gro, Versa Max Soybean, Versa MaxCorn, Ultra Che Corn Mix EDTA, ManniPlex for Beans, ManniPlex for Corn,Tracite LF Row Crop Mix, and KickStand Micro Mix. In one embodiment, themicronutrient product is Max In Ultra ZMB. In another embodiment, themicronutrient product is First Choice Foliar Nutrient. In yet anotherembodiment, the micronutrient product is Foli Gro. In one embodiment,the micronutrient product is Versa Max Soybean. In another embodiment,the micronutrient product is Versa Max Corn. In yet another embodiment,the micronutrient product is Ultra Che Corn Mix EDTA. In one embodiment,the micronutrient product is ManniPlex for Beans. In another embodiment,the micronutrient product is ManniPlex for Corn. In yet anotherembodiment, the micronutrient product is Tracite LF Row Crop Mix. In oneembodiment, the micronutrient product is KickStand Micro Mix.

In various embodiments, the composition comprises a ligand and afertilizer at specified ratios, for example volume/volume (v/v) ratios.In some embodiments, the ratio of ligand to fertilizer is about 8-64fluid ounces (fl. oz.) of ligand to about 1-10 gallons of fertilizer. Inother embodiments, the ratio of ligand to fertilizer is about 8-32 fl.oz. to about 1-5 gallons of fertilizer. In yet other embodiments, theratio of ligand to fertilizer is about 32-64 fl. oz. to about 5-10gallons of fertilizer.

In some embodiments, the ratio of ligand to fertilizer is about 8 fl.oz. of ligand to about 1 gallons of fertilizer. In other embodiments,the ratio of ligand to fertilizer is about 32 fl. oz. to about 5 gallonsof fertilizer. In yet other embodiments, the ratio of ligand tofertilizer is about 64 fl. oz. to about 10 gallons of fertilizer.

In other aspects, a composition consisting essentially of a ligand and afertilizer is provided. The various embodiments described with respectto the composition comprising a ligand and a fertilizer are alsoapplicable to the composition consisting essentially of a ligand and afertilizer.

In other aspects, a method of growing a plant is provided. The methodcomprises the step of contacting the plant with a composition comprisinga ligand. The various embodiments described with respect to thecomposition comprising a ligand and a fertilizer are also applicable tothe methods of growing a plant.

As used herein, the term “plant” refers to whole plants, plant organs(e.g., leaves, stems, roots, etc.), seeds, plant cells, propagules,embryos, and progeny of the same. In some embodiments, the plant is awhole plant. In other embodiments, the plant is a plant organ. In yetother embodiments, the plant is a seed. In some embodiments, the plantis a plant cell. Plant cells can be differentiated or undifferentiated(e.g., callus, suspension culture cells, protoplasts, leaf cells, rootcells, phloem cells, and pollen). In other embodiments, the plant is apropagule. In yet other embodiments, the plant is an embryo. In someembodiments, the plant is a plant progeny.

In some embodiments, the yield of the plant is improved via contactingthe plant with the composition. For example, the improvement in yieldcan be an improvement of the quantity of the plant (e.g., number ofbushels) produced per unit land measurement (e.g., acre or hectare)compared to a yield observed without contacting plants with thecomposition. In another exemplary embodiment, the improvement in yieldcan be a percentage improvement in the quantity of the plant producedper unit of land measurement compared to a yield observed withoutcontacting plants with the composition.

In some embodiments, the emergence of plants from seeds is improved viacontacting the plant with the composition. For example, the improvementin emergence can be evaluated based on the percentage of emergence ofplant from seeds compared to emergence observed without contacting seedswith the composition.

In various embodiments, the plant is corn (maize). In other embodiments,the plant is soybean. In yet other embodiments, the plant is wheat. Insome embodiments, the plant is sugarbeet. In various embodiments, theplant is a bean. In other embodiments, the bean is a dry edible bean. Inyet other embodiments, the plant is sorghum. In some embodiments, thesorghum is grain sorghum. In various embodiments, the plant is cotton.In other embodiments, the plant is potato. In some embodiments, theplant is barley. In other embodiments, the plant is sunflower.

In another aspect, a kit is provided. The kit comprises a ligand asdescribed herein. In this embodiment, the kit can further compriseinstructions for use, such as for combining the ligand with afertilizer, such as via tankmixing. The various embodiments describedwith respect to the composition comprising a ligand and a fertilizer arealso applicable to the kits described herein.

While the invention is susceptible to various modifications andalternative forms, specific embodiments are herein described in detail.It should be understood, however, that there is no intent to limit theinvention to the particular forms described, but on the contrary, theintention is to cover all modifications, equivalents, and alternativesfalling within the scope of the invention.

Example 1 Exemplary Ligands

For the examples disclosed herein, the compositions comprisedortho-ortho EDDHA as the exemplary ligand. The ortho-ortho EDDHA wasobtained from JAER (Laboratorio Jaer, S.A.) in Barcelona, Spain and wasutilized in the compositions according to following exemplaryembodiments.

Example 2 2012 Corn Trial—St. Cloud, Minn.

A corn trial utilizing a composition of the instant disclosure wasperformed in St. Cloud, Minn., in 2012. In this example, the compositioncomprised ortho-ortho EDDHA as the exemplary ligand.

In this example, Zea mays (variety DKC 43-48) seeds were planted on May15, 2012. Soil moisture was normal and the seed bed was compacted. Thetillage type was conventional till. The treated crop area for theinstant example was 300 square feet (ft²). The study design wasrandomized complete block (RCB) with four replications. A total of 13treatments were performed, as shown in Table 1.

TABLE 1 Treatment Descriptions. Treatment Treatment No. Type Name RateUnit Description 1 FERT 10-34-0 6 gal/a In Furrow 2 FERT 10-34-0 5.75gal/a In Furrow FERT EBMix 1 qt/a In Furrow 3 FERT 10-34-0 5.75 gal/a InFurrow FERT Blue Tsunami 1 qt/a In Furrow 4 FERT Redline 3 gal/a InFurrow FERT Water 3 gal/a In Furrow 5 FERT Redline 3 gal/a In FurrowFERT Water 2.9 gal/a In Furrow FERT Torque HC 1 oz/a In Furrow 6 FERTRedline 3 gal/a In Furrow FERT 10-34-0 3 gal/a In Furrow 7 FERT Water5.9 gal/a In Furrow FERT Ligand 8 oz/a In Furrow 8 FERT 10-34-0 5.95gal/a In Furrow FERT Ligand 8 oz/a In Furrow 9 FERT 10-34-0 5.7 gal/a InFurrow FERT Blue Tsunami 1 qt/a In Furrow FERT Ligand 8 oz/a In Furrow10 FERT 10-34-0 5.7 gal/a In Furrow FERT Blue Tsunami 1 qt/a In FurrowFERT Ligand 8 oz/a In Furrow FERT Torque HC 1.0 oz/a In Furrow 11 INSECapture LFR 8.5 oz/a In Furrow FERT Water 5.9 gal/a In Furrow 12 FERTRedline 3 gal/a In Furrow INSE Capture LFR 8.5 oz/a In Furrow FERT Water2.9 gal/a In Furrow 13 CHK Untreated Check

Corn plants were harvested on Oct. 10, 2012. Thereafter, the yield ofcorn plants administered the composition comprising ortho-ortho EDDHAwas compared to the yield of control corn plants that were untreated.Corn plants administered the composition comprising ortho-ortho EDDHAexhibited a yield increase of 8.5 bushels per acre compared to untreatedcontrols.

The yield of corn plants administered the composition comprisingortho-ortho EDDHA and further comprising 10-34-0 fertilizer viatankmixing was compared to the yield of corn plants that wereadministered 10-34-0 fertilizer alone. Corn plants administered thecomposition comprising ortho-ortho EDDHA plus 10-34-0 fertilizerexhibited a yield increase of 4 bushels per acre compared to corn plantsadministered 10-34-0 fertilizer alone.

The yield of corn plants administered the composition comprisingortho-ortho EDDHA and further comprising 10-34-0 fertilizer and achelated zinc (Blue Tsunami) via tankmixing was compared to the yield ofcorn plants that were administered 10-34-0 fertilizer and chelated zincvia tankmixing. Corn plants administered the composition comprisingortho-ortho EDDHA plus 10-34-0 fertilizer and chelated zinc exhibited ayield increase of 1.2 bushels per acre compared to corn plantsadministered 10-34-0 fertilizer and chelated zinc alone.

Example 3 2012 Soybean Trial—Rochester, Minn.

A soybean trial utilizing a composition of the instant disclosure wasperformed in Rochester, Minn., in 2012. In this example, the compositioncomprised ortho-ortho EDDHA as the exemplary ligand.

In this example, glycine max (variety Stine 19RA 02) soybean seeds wereplanted on May 9, 2012. The seed bed was smooth. The treated crop areafor the instant example was 300 square feet (ft²). The study design wasrandomized complete block (RCB) with four replications. A total of 10treatments were performed.

Soybean plants were harvested and, thereafter, the yield of soybeanplants administered the composition comprising ortho-ortho EDDHA andfurther comprising glyphosate (Buccaneer Plus) and a water conditioningagent (N-Tense) via tankmixing was compared to the yield of soybeanplants that were administered glyphosate and a water conditioning agentvia tankmixing. Soybean plants administered the composition comprisingortho-ortho EDDHA plus glyphosate and a water conditioning agentexhibited a yield increase of 5.7 bushels per acre compared to soybeanplants administered glyphosate and a water conditioning agent alone.

Example 4 2013 Corn Trial—Casselton, N. Dak.

A corn trial utilizing a composition of the instant disclosure wasperformed near Chaffee, N. Dak., in 2013. In this example, thecomposition comprised ortho-ortho EDDHA as the exemplary ligand.

Planting was arranged in a randomized complete block design with fourreplications. Individual treatment plots measured 11 feet wide and 30feet long. Due to dry conditions in spring 2013, there was some unevengermination but overall plant stands were good.

In this example, corn variety Pioneer 8640 Roundup Ready was planted onMay 13, 2013 using a John Deere MaxEmerge II planter. Corn was planted 2inches deep to a population of 35,000 plants per acre, and a 22-inch rowspacing was used. Roundup herbicide was applied once for weed control.Plots were not cultivated and some late hand labor was used as neededfor weed control.

Soil nitrogen levels were adjusted with fertilizer to approximately 200lbs/acre of available residual soil test plus added fertilizer N. Atotal of 8 treatments were performed, as shown in Table 2.

TABLE 2 Treatment Descriptions. Treatment Treatment No. Type Name RateUnit Description 1 CHK Untreated Check 2 FERT 10-34-0 5 gal/a In Furrow3 FERT Liqand 3 gal/a In Furrow 4 FERT 10-34-0 5 gal/a In Furrow FERTBlue Tsunami 1 qt/a In Furrow 5 FERT Liqand 8 gal/a In Furrow 6 FERT10-34-0 5 gal/a In Furrow FERT WC150 In Furrow 7 FERT 10-34-0 5 gal/a InFurrow FERT Blue Tsunami 1 qt/a In Furrow FERT WC150 In Furrow 8 FERT10-34-0 5 gal/a In Furrow FERT WC150 In Furrow

The yield of corn plants administered the composition comprisingortho-ortho EDDHA was compared to the yield of control corn plants thatwere untreated. Corn plants administered the composition comprisingortho-ortho EDDHA exhibited a yield increase of 4.6 bushels per acrecompared to untreated controls.

The yield of corn plants administered the composition comprisingortho-ortho EDDHA and further comprising 10-34-0 fertilizer viatankmixing was compared to the yield of corn plants that wereadministered 10-34-0 fertilizer alone. Corn plants administered thecomposition comprising ortho-ortho EDDHA plus 10-34-0 fertilizerexhibited a yield increase of 25.4 bushels per acre compared to cornplants administered 10-34-0 fertilizer alone.

The yield of corn plants administered the composition comprisingortho-ortho EDDHA and further comprising 10-34-0 fertilizer and achelated zinc (Blue Tsunami) via tankmixing was compared to the yield ofcorn plants that were administered 10-34-0 fertilizer and chelated zincvia tankmixing. Corn plants administered the composition comprisingortho-ortho EDDHA plus 10-34-0 fertilizer and chelated zinc exhibited ayield increase of 34.1 bushels per acre compared to corn plantsadministered 10-34-0 fertilizer and chelated zinc alone.

Example 5 2013 Corn Trial—Brookings, S. Dak.

A corn trial utilizing a composition of the instant disclosure wasperformed near Brookings, S. Dak., in 2013. In this example, thecomposition comprised ortho-ortho EDDHA as the exemplary ligand.

In this example, corn variety Dekalb 45-51 was planted on May 14, 2013.Corn was planted to a population of 32,000 plants per acre, and a30-inch row spacing was used. The soil type was Brandt silty clay loam.A total of 8 treatments were performed, as shown in Table 3.

TABLE 3 Treatment Descriptions. Treatment Treatment No. Type Name RateUnit Description 1 CHK Untreated Check 2 FERT 10-34-0 5 gal/a In Furrow3 FERT WC139 3 gal/a In Furrow 4 FERT 10-34-0 5 gal/a In Furrow FERTBlue Tsunami 1 qt/a In Furrow 5 FERT Ligand 8 oz/a In Furrow 6 FERT10-34-0 5 gal/a In Furrow FERT Ligand 8 oz/a In Furrow 7 FERT 10-34-0 5gal/a In Furrow FERT Blue Tsunami 1 qt/a In Furrow FERT Ligand 8 oz/a InFurrow 8 FERT WC139 3 gal/a In Furrow FERT WC171 0.1 oz/a In Furrow

Corn plants were harvested on Oct. 24, 2013. Thereafter, the yield ofcorn plants administered the composition comprising ortho-ortho EDDHAwas compared to the yield of control corn plants that were untreated.Corn plants administered the composition comprising ortho-ortho EDDHAexhibited a yield increase of 8.2 bushels per acre compared to untreatedcontrols.

The yield of corn plants administered the composition comprisingortho-ortho EDDHA and further comprising 10-34-0 fertilizer and achelated zinc (Blue Tsunami) via tankmixing was compared to the yield ofcorn plants that were administered 10-34-0 fertilizer and chelated zincvia tankmixing. Corn plants administered the composition comprisingortho-ortho EDDHA plus 10-34-0 fertilizer and chelated zinc exhibited ayield increase of 20.6 bushels per acre compared to corn plantsadministered 10-34-0 fertilizer and chelated zinc alone.

Example 6 2013 Soybean Trial—St. Cloud, Minn.

A soybean trial utilizing a composition of the instant disclosure wasperformed in St. Cloud, Minn., in 2013. In this example, the compositioncomprised ortho-ortho EDDHA as the exemplary ligand.

In this example, glycine max (variety PB0954RR) soybean seeds wereplanted on Jun. 17, 2013. The tillage type was conventional till. Thetreated crop area for the instant example was 300 square feet (ft²). Thestudy design was randomized complete block (RCB) with four replications.A total of 6 treatments were performed, as shown in Table 4.

TABLE 4 Treatment Descriptions. Treatment Treatment No. Type Name RateUnit Description 1 CHK Untreated Check 2 FERT WC139 2 gal/a In Furrow 3FERT WC139 3 gal/a In Furrow 4 FERT Ligand 12 oz/a In Furrow 5 FERTWC150 2 lb/a In Furrow 6 FERT 10-34-0 2 gal/a In Furrow

Soybean plants were harvested on Oct. 11, 2013. Thereafter, yield ofsoybean plants administered the composition comprising ortho-ortho EDDHAwas compared to the yield of control soybean plants that were untreated.Soybean plants administered the composition comprising ortho-ortho EDDHAexhibited a yield increase of 5.1 bushels per acre compared to untreatedcontrols.

Example 7 2013 Soybean Trial—Rochester, Minn.

A soybean trial utilizing a composition of the instant disclosure wasperformed in Rochester, Minn., in 2013. In this example, the compositioncomprised ortho-ortho EDDHA as the exemplary ligand.

In this example, DuPont (Pioneer 92Y22) soybean seeds were planted onMay 16, 2013. The seed bed was medium. The treated crop area for theinstant example was 250 square feet (ft²). The study design wasrandomized complete block (RCB) with four replications.

Soybean plants were harvested and, thereafter, the yield of soybeanplants administered the composition comprising ortho-ortho EDDHA andfurther comprising glyphosate (Buccaneer Plus) and a water conditioningagent (N-Tense) via tankmixing was compared to the yield of soybeanplants that were administered glyphosate and a water conditioning agentvia tankmixing. Soybean plants administered the composition comprisingortho-ortho EDDHA plus glyphosate and a water conditioning agentexhibited a yield increase of 2 bushels per acre compared to soybeanplants administered glyphosate and a water conditioning agent alone.

Example 8 2013 Soybean Trial—Arlington, Wis.

A soybean trial utilizing a composition of the instant disclosure wasperformed in Arlington, Wis., in 2013. In this example, the compositioncomprised ortho-ortho EDDHA as the exemplary ligand.

In this example, glycine max (variety Asgrow 2130) soybean seeds wereplanted on Jun. 1, 2013. The tillage type was conventional till. Thetreated crop area for the instant example was 250 square feet (ft²). Thestudy design was randomized complete block (RCB) with four replications.A total of 12 treatments were performed.

Soybean plants were harvested and, thereafter, the yield of soybeanplants administered the composition comprising ortho-ortho EDDHA andfurther comprising glyphosate (Buccaneer Plus) and a water conditioningagent (N-Tense) via tankmixing was compared to the yield of soybeanplants that were administered glyphosate and a water conditioning agentvia tankmixing. Soybean plants administered the composition comprisingortho-ortho EDDHA plus glyphosate and a water conditioning agentexhibited a yield increase of 8 bushels per acre compared to soybeanplants administered glyphosate and a water conditioning agent alone.

Example 9 2013 Corn Seed Trial—Brookings, S. Dak.

A corn seed trial utilizing a composition of the instant disclosure wasperformed in Brookings, S. Dak., in 2013. In this example, thecomposition comprised ortho-ortho EDDHA as the exemplary ligand (i.e.,“WC101”).

The study design was randomized complete block (RCB) with fourreplications. As shown in Table 5, a total of 3 treatments wereperformed in 4 plots for each treatment group.

TABLE 5 Treatment Descriptions. Treatment Treatment Plot No. Plot No.Plot No. Plot No. No. Type Name (Rep 1) (Rep 2) (Rep 3) (Rep 4) 1 CHKUntreated 101 201 301 401 Check 2 Seed WC101 102 202 302 402 Treatment 3Seed WC101 + 103 203 303 403 Treatment WC143

Plot unit sizes were approximately 10 feet by 40 feet according to thedisclosed layout of FIG. 9.

The yield of corn seeds administered the composition comprisingortho-ortho EDDHA was compared to the yield of control corn seeds thatwere untreated. Treatment of corn seeds administered the compositioncomprising ortho-ortho EDDHA exhibited a yield increase of 1.4 bushelsper acre compared to untreated controls.

In addition, the emergence of corn seeds administered the compositioncomprising ortho-ortho EDDHA was compared to the emergence of controlcorn seeds that were untreated. Treatment of corn seeds administered thecomposition comprising ortho-ortho EDDHA exhibited an emergence increaseby 3.5% compared to untreated controls.

The yield of corn seeds administered the composition comprisingortho-ortho EDDHA and further comprising a micronutrient product wascompared to the yield of control corn seeds that were untreated.Treatment of corn seeds administered the composition comprisingortho-ortho EDDHA and the micronutrient product exhibited a yieldincrease of 9.5 bushels per acre compared to untreated controls.

Furthermore, the emergence of corn seeds administered the compositioncomprising ortho-ortho EDDHA and further comprising a micronutrientproduct (WC143) was compared to the emergence of control corn seeds thatwere untreated. Treatment of corn seeds administered the compositioncomprising ortho-ortho EDDHA and the micronutrient product exhibited anemergence increase by 5.5% compared to untreated controls.

Example 10 2014 Corn Trial—Scandia, Kans.

A corn trial utilizing a composition of the instant disclosure wasperformed near Scandia, Kans., in 2014. In this example, the compositioncomprised ortho-ortho EDDHA as the exemplary ligand (i.e., “WC101”).

In this example, corn was planted on May 2, 2014. Treatments wereapplied as starter applications at planting with an application volumeof 6 gpa. Treatments were performed as shown in Table 6. Weed controlwas completed as needed to keep weed free with maintenance sprays.

TABLE 6 Treatment Descriptions. Treatment Treatment No. Type Name RateUnit Description 1 CHK Untreated 0 Check 2 FERT 10-34-0 5 gal/a InFurrow 3 FERT WC139 3 gal/a In Furrow 4 FERT 10-34-0 5 gal/a In FurrowFERT Blue Tsunami 1 qt/a In Furrow 5 FERT 10-34-0 5 gal/a In Furrow FERTLigand 24 oz/a In Furrow 6 FERT Local P Starter 3 gal/a In Furrow(9-18-9) 7 FERT Local P Starter 3 gal/a In Furrow (9-18-9) FERT Ligand24 oz/a In Furrow 8 FERT 10-34-0 5 gal/a In Furrow FERT Aventine 2 qt/aIn Furrow 9 FERT 10-34-0 5 gal/a In Furrow FERT Blue Tsunami 1 qt/a InFurrow FERT WC101 24 oz/a In Furrow 10 FERT 10-34-0 5 gal/a In FurrowFERT Aventine 2 qt/a In Furrow FERT WC101 24 oz/a In Furrow 11 FERTWC150 8 oz/a In Furrow 12 FERT 10-34-0 5 gal/a In Furrow FERT BlueTsunami 1 qt/a In Furrow FERT WC150 8 oz/a In Furrow

Preliminary soil test values were collected for each replication andindicated a low soil test P that should be conducive to corn response tostarter P application. Other parameters including K, Zn and soil pH wereevaluated to be at optimum condition, and there was no need foradditional fertilization. The soil tests by replication in the studyarea are shown in Table 7.

Data collected included yield (bu/acre), test weight, moisture,preliminary soil analysis, and plant tissue and grain analysis. Grainyield was measured harvesting the two center rows with a plot combine.Total phosphorus (P) content in plant tissue was measured for the earleaf and grain P concentration was measured after harvest with grainanalysis for total P content.

TABLE 7 Soil tests by replication in the study area. Mehlich P K OM ZnReplication pH (ppm) (ppm) (%) (ppm) 1 6.37 12.5 600 2.9 2.1 2 6.28 13.1630 3.0 2.2 3 6.44 15.2 640 3.0 2.3 4 6.35 14.1 670 3.0 2.6 Average 6.3614.1 670 3.0 2.3

Fertilizer treatment mixes were completed before application. Visualobservations in some instances indicated possible compatibility issueswith WC101 product and various fertilizers, for instance 9-18-9.Although treatment application was complicated in some instances, therates and placement for the instant example were believed to be withinacceptable parameters. Grain yield was measured harvesting the twocenter rows with a plot combine. Total phosphorus (P) content in planttissue was measured for the ear leaf and grain P concentration wasmeasured after harvest with grain analysis for total P content.

As shown in Table 8, each evaluated parameter demonstrated astatistically significant effect for ligand-treated plants compared tocontrol treatment.

TABLE 8 Statistical significance of treatment effects. Type III Tests ofFixed Effects Effect Num DF Den DF F Value Pr > F Yield 11 33 2.80 0.011Tissue P 11 33 1.94 0.070 Grain P Removal 11 33 2.75 0.012

As shown in FIG. 1, grain yield increased approximately 25 bushels peracre with the highest yielding treatment compared to control treatment.Furthermore, FIG. 2 and FIG. 3 demonstrate superior effects for tissue Puptake and grain P uptake, respectfully, compared to control treatment.As shown in FIGS. 1-3, inclusion of WC101 in the treatment groupsresulted in an improvement for each evaluated parameter, especially fortreatment groups that do not include micronutrients (e.g., 10-34-0 and9-18-9).

Example 11 Laboratory Soil Micronutrient Recovery Experiment

A soil micronutrient recovery trial utilizing a composition of theinstant disclosure was performed in the laboratory. In this example, thecomposition comprised ortho-ortho EDDHA as the exemplary ligand (i.e.,“WC101”).

The objective of the instant example was to demonstrate the percentagerecovery (i.e., % availability) over time of micronutrients present invarious soil types. Three soil types were tested: (1) Pullman series,(2) Amarillo series, and (3) Olton series. The soil types are alkaline(pH>7.5) semi-arid soils and are often calcareous in nature.Furthermore, the chemistry of these soils indicate that micronutrientscould be tied up easily by soil constituents, thereby reducingmicronutrient availability to plants.

Approximately 500 grams of each soil type was incubated in a plasticcontainer. WC101 was applied to the soil types, and treated soils wereincubated for approximately 60 days and sampled at 2-3 weeks intervals.DTPA extraction techniques was used to extract plant availablemicronutrients (Fe, Cu, Mn, and Zn). Plant available soil P wasdetermined in Mehlich-3 extracts. Elemental analysis was conducted usingthe ICP.

FIGS. 4-8 show the effects of WC101 treatment on the various soil types.In FIG. 4, WC101 treatment was effective to increase the percentage ofcopper (Cu) recovery 35 days after the addition of WC101 to the soiltypes. In FIG. 5, WC101 treatment was effective to increase thepercentage of iron (Fe) recovery 35 days after the addition of WC101 tothe soil types. In FIG. 6, WC101 treatment was effective to increase thepercentage of manganese (Mn) recovery 35 days after the addition ofWC101 to the soil types. In FIG. 7, WC101 treatment was effective toincrease the percentage of zinc (Zn) recovery 35 days after the additionof WC101 to the soil types. In FIG. 8, WC101 treatment was effective toincrease the percentage of phosphorus (P) recovery 35 days after theaddition of WC101 to the soil types.

What is claimed is:
 1. A method of growing a plant, said methodcomprising the step of contacting the plant with a compositioncomprising a ligand, wherein the ligand is ortho-ortho EDDHA, whereinthe ligand is non-chelated, and wherein the composition is substantiallyfree of iron.
 2. The method of claim 1 wherein the yield of the plant isimproved via contacting the plant with the composition.
 3. The method ofclaim 1 wherein the emergence of plants from seeds is improved viacontacting the plant with the composition.
 4. The method of claim 1wherein the composition further comprises a fertilizer.
 5. The method ofclaim 1 wherein the composition further comprises zinc.
 6. The method ofclaim 5 wherein the zinc is chelated zinc.
 7. The method of claim 1wherein the composition further comprises a herbicide.
 8. The method ofclaim 7 wherein the herbicide is selected from the group consisting of2,4-D, 2,4-DB, acetochlor, acifluorfen, alachlor, ametryn, atrazine,aminopyralid, benefin, bensulfuron, bensulide, bentazon, bromacil,bromoxynil, butylate, carfentrazone, chlorimuron, chlorsulfuron,clethodim, clomazone, clopyralid, cloransulam, cycloate, DCPA,desmedipham, dicamba, dichlobenil, diclofop, diclosulam, diflufenzopyr,dimethenamid, diquat, diuron, DSMA, endothall, EPTC, ethalfluralin,ethofumesate, fenoxaprop, fluazifop-P, flucarbazone, flufenacet,flumetsulam, flumiclorac, flumioxazin, fluometuron, fluroxypyr,fomesafen, foramsulfuron, glufosinate, glyphosate, halosulfuron,hexazinone, imazamethabenz, imazamox, imazapic, imazaquin, imazethapyr,isoxaben, isoxaflutole, lactofen, linuron, MCPA, MCPB, mesotrione,metolachlor-s, metribuzin, metsulfuron, molinate, MSMA, napropamide,naptalam, nicosulfuron, norflurazon, oryzalin, oxadiazon, oxyfluorfen,paraquat, pelargonic acid, pendimethalin, phenmedipham, picloram,primisulfuron, prodiamine, prometryn, pronamide, propanil, prosulfuron,pyrazon, pyrithiobac, quinclorac, quizalofop, rimsulfuron, sethoxydim,siduron, simazine, sulfentrazone, sulfometuron, sulfosulfuron,tebuthiuron, terbacil, thiazopyr, thifensulfuron, thiobencarb,tralkoxydim, triallate, triasulfuron, tribenuron, triclopyr,trifluralin, triflusulfuron, and any combination thereof.
 9. The methodof claim 7 wherein the herbicide is glyphosate.
 10. The method of claim1 wherein the composition further comprises a water conditioning agent.11. The method of claim 1 wherein the composition further comprises amicronutrient product.
 12. The method of claim 11 wherein themicronutrient product comprises one or more trace elements selected fromthe group consisting of copper, manganese, zinc, cobalt, molybdenum, andboron.
 13. The method of claim 4 wherein the composition consistsessentially of the ligand and the fertilizer.
 14. The method of claim 4wherein the fertilizer is an ammonium polyphosphate fertilizer.
 15. Themethod of claim 13 wherein the fertilizer is an ammonium polyphosphatefertilizer.
 16. The method of claim 14 wherein the ammoniumpolyphosphate fertilizer is ammonium polyphosphate 10-34-0 fertilizer.17. The method of claim 1 wherein the plant is a seed.
 18. The method ofclaim 1 wherein the plant is selected from the group consisting of corn,soybean, wheat, sugarbeet, bean, sorghum, cotton, potato, barley andsunflower.
 19. The method of claim 1 wherein the plant is corn.
 20. Themethod of claim 1 wherein the plant is soybean.